{"id":4013,"date":"2025-12-23T14:28:30","date_gmt":"2025-12-23T06:28:30","guid":{"rendered":"https:\/\/ramiefb.meealldemo.com\/?post_type=blog&#038;p=4013"},"modified":"2025-12-23T14:28:30","modified_gmt":"2025-12-23T06:28:30","slug":"main-content-of-the-study","status":"publish","type":"blog","link":"https:\/\/www.antsjapan.com\/zh\/blog\/main-content-of-the-study\/","title":{"rendered":"\u300a\u5173\u4e8e\u82ce\u9ebbBnGCL1\u57fa\u56e0\u5728\u5e94\u5bf9\u5e72\u65f1\u80c1\u8feb\u4e2d\u7684\u529f\u80fd\u7814\u7a76\u300b\u7684\u4e3b\u8981\u5185\u5bb9"},"content":{"rendered":"<h2 class=\"wp-block-heading\">I. \u7814\u7a76\u80cc\u666f\u4e0e\u76ee\u6807<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5e72\u65f1\u662f\u5f71\u54cd\u690d\u7269\u751f\u957f\u7684\u4e3b\u8981\u975e\u751f\u7269\u80c1\u8feb\uff0c\u5b83\u4f1a\u6291\u5236\u751f\u957f\u3001\u964d\u4f4e\u5149\u5408\u4f5c\u7528\u6548\u7387\u5e76\u5f15\u53d1\u4ee3\u8c22\u7d0a\u4e71\u3002\u4f5c\u4e3a\u4e2d\u56fd\u7279\u6709\u7684\u97e7\u76ae\u7ea4\u7ef4\u4f5c\u7269\uff0c\u82ce\u9ebb\uff08Boehmeria nivea L.\uff09\u5177\u6709\u5f88\u5f3a\u7684\u6297\u65f1\u6027\uff0c\u4f46\u5e72\u65f1\u4ecd\u4f1a\u5bf9\u5176\u4ea7\u91cf\u548c\u54c1\u8d28\u4ea7\u751f\u5f71\u54cd\u3002.<\/li>\n\n\n\n<li>\u8c37\u6c28\u9178-\u534a\u80f1\u6c28\u9178\u8fde\u63a5\u9176\uff08GCL\uff09\uff0c\u53c8\u79f0\u03b3-\u8c37\u6c28\u9170\u534a\u80f1\u6c28\u9178\u5408\u6210\u9176\uff08\u03b3-GCS\uff09\uff0c\u50ac\u5316\u8c37\u80f1\u7518\u80bd\uff08GSH\uff09\u7684\u751f\u7269\u5408\u6210\u3002 GSH\u5728\u690d\u7269\u7684\u6297\u6c27\u5316\u9632\u5fa1\u548c\u7ef4\u6301\u6c27\u5316\u8fd8\u539f\u7a33\u6001\u65b9\u9762\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\uff0c\u4f46\u5173\u4e8e\u690d\u7269GCL\u57fa\u56e0\u7684\u7814\u7a76\u4ecd\u8f83\u4e3a\u6709\u9650\u3002.<\/li>\n\n\n\n<li>\u672c\u7814\u7a76\u65e8\u5728\u63a2\u8ba8\u82ce\u9ebbBnGCL1\u57fa\u56e0\u5728\u5e72\u65f1\u80c1\u8feb\u4e0b\u7684\u529f\u80fd\u673a\u5236\uff0c\u4e3a\u82ce\u9ebb\u6297\u65f1\u5206\u5b50\u673a\u5236\u7814\u7a76\u53ca\u6297\u65f1\u54c1\u79cd\u80b2\u79cd\u63d0\u4f9b\u7406\u8bba\u4f9d\u636e\u548c\u5019\u9009\u57fa\u56e0\u3002.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">II. \u7814\u7a76\u6750\u6599\u4e0e\u65b9\u6cd5<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">(I) \u5b9e\u9a8c\u6750\u6599<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u82ce\u9ebb\u54c1\u79cd\uff1a\u4e2d\u73e01\u53f7\uff1b\u62df\u5357\u82a5\uff08Arabidopsis thaliana\uff09Col-0\u53ca\u76f8\u5173\u8f7d\u4f53\uff08pCAMBIA1300-35S-BnGCL1-myc\u7b49\uff09\u4fdd\u5b58\u5728\u5b9e\u9a8c\u5ba4\u4e2d\u3002.<\/li>\n\n\n\n<li>Agrobacterium tumefaciens GV3101 \u4ee5\u53ca\u5927\u80a0\u6746\u83cc\u83cc\u682a TOP10\u3001DH5\u03b1 \u5747\u8d2d\u81ea\u4e0a\u6d77\u4f1f\u8fea\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8\u3002.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">(II) \u6838\u5fc3\u7814\u7a76\u65b9\u6cd5<\/h3>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li>\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\uff1a\u5229\u7528NCBI\u548cExPASy\u7b49\u5de5\u5177\uff0c\u5bf9BnGCL1\u57fa\u56e0\u7684\u5f00\u653e\u9605\u8bfb\u6846\uff08ORF\uff09\u3001\u86cb\u767d\u8d28\u7684\u7406\u5316\u6027\u8d28\u3001\u4fdd\u5b88\u7ed3\u6784\u57df\u4ee5\u53ca\u7269\u79cd\u540c\u6e90\u6027\u8fdb\u884c\u4e86\u5206\u6790\u3002.<\/li>\n\n\n\n<li>\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u5206\u6790\uff1a\u91c7\u752820% PEG-200\u6a21\u62df\u5e72\u65f1\u80c1\u8feb\uff0c\u5e76\u901a\u8fc7qPCR\u68c0\u6d4b\u4e0d\u540c\u65f6\u95f4\u70b9\u82ce\u9ebb\u53f6\u7247\u4e2dBnGCL1\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\u3002.<\/li>\n\n\n\n<li>\u8fc7\u8868\u8fbe\u9a8c\u8bc1\uff1a\u6784\u5efa\u4e86\u8fc7\u8868\u8fbe\u8f7d\u4f53\u5e76\u5c06\u5176\u8f6c\u5316\u81f3\u62df\u5357\u82a5\u4e2d\u3002\u7b5b\u9009\u51fa\u9633\u6027\u7cfb\u540e\uff0c\u6d4b\u5b9a\u4e86\u690d\u7269\u5728\u5e72\u65f1\u5e94\u6fc0\u6761\u4ef6\u4e0b\u7684\u8868\u578b\u3001\u751f\u7406\u6307\u6807\u4ee5\u53ca\u6297\u65f1\u76f8\u5173\u57fa\u56e0\u7684\u8868\u8fbe\u60c5\u51b5\u3002.<\/li>\n\n\n\n<li>\u57fa\u56e0\u6c89\u9ed8\u9a8c\u8bc1\uff1a\u5229\u7528pTRV-VIGS\u7cfb\u7edf\u5bf9\u82ce\u9ebb\u7684BnGCL1\u57fa\u56e0\u8fdb\u884c\u6c89\u9ed8\uff0c\u5e76\u89c2\u5bdf\u4e86\u5e72\u65f1\u80c1\u8feb\u4e0b\u690d\u7269\u8868\u578b\u53caMDA\u542b\u91cf\u7684\u53d8\u5316\u3002.<\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\">III. \u6838\u5fc3\u7814\u7a76\u6210\u679c<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">(I) BnGCL1\u57fa\u56e0\u7684\u7279\u5f81<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u57fa\u56e0\u5e8f\u5217\uff1a\u5f00\u653e\u9605\u8bfb\u6846\uff08ORF\uff09\u957f\u5ea6\u4e3a1581 bp\uff0c\u7f16\u7801526\u4e2a\u6c28\u57fa\u9178\uff1b\u8be5\u86cb\u767d\u8d28\u7684\u7b49\u7535\u70b9\u4e3a5.79\uff0c\u5206\u5b50\u91cf\u4e3a59,123.98 Da\uff0c\u662f\u4e00\u79cd\u7a33\u5b9a\u7684\u86cb\u767d\u8d28\uff0c\u5e76\u542b\u6709\u4e00\u4e2a\u4fdd\u5b88\u7684PLN02611\u7ed3\u6784\u57df\u3002.<\/li>\n\n\n\n<li>\u540c\u6e90\u6027\uff1a\u4e0e\u70df\u8349\uff08Nicotiana tabacum\uff09\u3001\u62df\u5357\u82a5\uff08Arabidopsis thaliana\uff09\u53ca\u5176\u4ed6\u690d\u7269\u7684\u6c28\u57fa\u9178\u5e8f\u5217\u76f8\u4f3c\u5ea6\u8d85\u8fc780%\uff0c\u4e14\u4e0e\u62df\u5357\u82a5\u548c\u82a5\u83dc\uff08Brassica juncea\uff09\u7684\u9057\u4f20\u5173\u7cfb\u6700\u4e3a\u5bc6\u5207\u3002.<\/li>\n\n\n\n<li>\u8868\u8fbe\u6a21\u5f0f\uff1a\u8be5\u57fa\u56e0\u5728\u82ce\u9ebb\u7684\u6839\u3001\u830e\u548c\u53f6\u4e2d\u5747\u6709\u8868\u8fbe\uff0c\u5176\u8868\u8fbe\u6c34\u5e73\u5728\u5e72\u65f1\u5e94\u6fc012\u5c0f\u65f6\u540e\u8fbe\u5230\u5cf0\u503c\uff0c\u662f\u5904\u7406\u524d\u76843.62\u500d\u3002.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">(II) BnGCL1\u8fc7\u8868\u8fbe\u7684\u6297\u65f1\u6548\u5e94<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u8868\u578b\uff1a \u5728\u5e72\u65f1\u80c1\u8feb\u6761\u4ef6\u4e0b\uff0c\u8fc7\u8868\u8fbe\u7684\u62df\u5357\u82a5\u6839\u957f\u589e\u52a0\u4e8628.92%-57.23%\uff0c\u9c9c\u91cd\u589e\u52a0\u4e8681.42%-131.19%\uff0c\u751f\u957f\u72b6\u51b5\u4f18\u4e8e\u91ce\u751f\u578b\u3002.<\/li>\n\n\n\n<li>\u5149\u5408\u8272\u7d20\uff1a\u53f6\u7eff\u7d20a\u7684\u542b\u91cf\u589e\u52a0\u4e8625.07%-99.09%\uff0c\u53f6\u7eff\u7d20b\u7684\u542b\u91cf\u589e\u52a0\u4e8611.14%-47.26%\uff0c\u4ece\u800c\u4f7f\u5149\u5408\u80fd\u529b\u66f4\u52a0\u7a33\u5b9a\u3002.<\/li>\n\n\n\n<li>\u751f\u7406\u6307\u6807\uff1aAPX\u548c\u03b3-GCL\u7b49\u6297\u6c27\u5316\u9176\u7684\u6d3b\u6027\u663e\u8457\u589e\u52a0\uff0cGSSG\u542b\u91cf\u5347\u9ad8\uff0cSOD\u6d3b\u6027\u5f97\u5230\u6539\u5584\uff0c\u4ece\u800c\u589e\u5f3a\u4e86\u6e05\u9664\u6d3b\u6027\u6c27\u7684\u80fd\u529b\uff1bPro\u548cGSH\u7684\u542b\u91cf\u4e0b\u964d\uff0c\u63a8\u6d4b\u8fd9\u4e0e\u4ee3\u8c22\u91cd\u5206\u5e03\u6216\u6d88\u8017\u6709\u5173\u3002.<\/li>\n\n\n\n<li>\u57fa\u56e0\u8868\u8fbe\uff1a\u5b83\u663e\u8457\u4e0a\u8c03\u4e86AtGST1\u3001AtGST11\u3001AtNCED3\u548cAtWRKY40\u7b49\u6297\u65f1\u76f8\u5173\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u6d89\u53ca\u6297\u6c27\u5316\u548cABA\u4fe1\u53f7\u4f20\u5bfc\u901a\u8def\u3002.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">(III) \u6c89\u9ed8BnGCL1\u7684\u5f71\u54cd<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5728\u5e72\u65f1\u80c1\u8feb\u6761\u4ef6\u4e0b\uff0c\u57fa\u56e0\u6c89\u9ed8\u54c1\u7cfb\u7684\u53f6\u7247\u51fa\u73b0\u66f4\u660e\u663e\u7684\u840e\u852b\uff0c\u4e14MDA\u542b\u91cf\u663e\u8457\u9ad8\u4e8e\u5bf9\u7167\u7ec4\uff0c\u8868\u660e\u5176\u6297\u65f1\u80fd\u529b\u663e\u8457\u4e0b\u964d\uff0c\u8fd9\u8bc1\u5b9e\u4e86BnGCL1\u7684\u6297\u65f1\u529f\u80fd\u3002.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">IV. \u7814\u7a76\u7ed3\u8bba<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">BnGCL1\u57fa\u56e0\u901a\u8fc7\u589e\u5f3a\u6297\u6c27\u5316\u9176\u6d3b\u6027\u3001\u8c03\u8282\u6e17\u900f\u8c03\u8282\u7269\u8d28\u7684\u4ee3\u8c22\u4ee5\u53ca\u4e0a\u8c03\u6297\u65f1\u76f8\u5173\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u53c2\u4e0e\u690d\u7269\u7684\u6297\u65f1\u5e94\u6fc0\u53cd\u5e94\uff0c\u4ece\u800c\u663e\u8457\u63d0\u9ad8\u690d\u7269\u7684\u6297\u65f1\u6027\u3002 \u8be5\u57fa\u56e0\u4e0d\u4ec5\u4e3a\u6297\u65f1\u82ce\u9ebb\u7684\u5206\u5b50\u80b2\u79cd\u63d0\u4f9b\u4e86\u91cd\u8981\u7684\u5019\u9009\u57fa\u56e0\uff0c\u4e5f\u4e3a\u5176\u4ed6\u4f5c\u7269\u7684\u6297\u65f1\u6027\u6539\u826f\u63d0\u4f9b\u4e86\u53c2\u8003\u3002.<\/p>","protected":false},"template":"","class_list":["post-4013","blog","type-blog","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.antsjapan.com\/zh\/wp-json\/wp\/v2\/blog\/4013","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.antsjapan.com\/zh\/wp-json\/wp\/v2\/blog"}],"about":[{"href":"https:\/\/www.antsjapan.com\/zh\/wp-json\/wp\/v2\/types\/blog"}],"wp:attachment":[{"href":"https:\/\/www.antsjapan.com\/zh\/wp-json\/wp\/v2\/media?parent=4013"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}